A) By supporting art, wealthy merchant families created an environment that also promoted cultural achievements.
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C. Your audience
Your audience should determine whether your tone is formal or casual. The audience's expectations for the speech can determine the tone. If the speech is meant to be lighthearted and informal, the tone of the speech must be casual. A formal tone would be used in an academic setting.
Vacuoles are membrane-bound structures found in both animal and plant cells.
They have three important functions in plants -- provide support or rigidity, a storage for nutrients and waste matter until it can be removed, and decompose complex molecules.